Garden Wall House / Sarah Kahn Architect

first_imgArchitects: Sarah Kahn Architect Area Area of this architecture project “COPY” Garden Wall House / Sarah Kahn Architect Manufacturers: Lysaght, Radial Timbers, UnilamSave this picture!© Tatjana PlittRecommended ProductsWindowsJansenWindows – Janisol PrimoEnclosures / Double Skin FacadesRodecaRound Facade at Omnisport Arena ApeldoornWoodBruagBalcony BalustradesWoodAccoyaAccoya® Cladding, Siding & FacadesText description provided by the architects. The project involved the insertion of a new 3-bedroom residence as a second dwelling on the lot, adjacent to an existing garage on large block in Carlton North.Save this picture!© Tatjana PlittThe block itself was a complex site with the existing garage and studio building to one edge surrounded by lane ways, a large double-storey heritage terrace house facing the principal street front to the eastern side and the remaining land an overgrown, rambling garden that was too large to be properly maintained by the owner and lacked connection to the main house. The owner wanted a simple, flexible and sunlit home, in which she could remain living alone happily and safely well into old-age, to be inserted into the unused garden space.Save this picture!SectionsThe new home had to sit alongside the existing studio/garage building, allow for direct entry to the second dwelling from the lane way plus maintain access into the garage and to the studio above whilst retaining sunlight in the existing studio room, where the owner worked. A keen gardener, she also wanted to maximize connection to the outdoors and retain some of the established planting, but create a more manageable garden.Save this picture!© Tatjana PlittThe new house also had to sit sympathetically into the varied, lane way streetscape and respond to the requirements of the heritage overlay. The project also had a very tight budget to achieve the desired areas, so we had to plan the building as efficiently as possible and keep the structure and built form very simple. Integration of materials salvaged from the existing garden area, including bricks and bluestone pavers, was also a critical concern.Save this picture!© Tatjana PlittWe approached the design by breaking up the site and creating a ‘  patchwork’   of indoor and outdoor spaces, public and private zones, and small garden zones.Save this picture!Ground Floor PlanThe ground floor pavilion of the new house is framed by a recycled red brick wall, which extends beyond the enclosure of house to embrace the garden and separates the home from the lane way, creating a private garden sanctuary.A new public entry porch zone was created to the south of the new house which separates the new dwelling from the existing garage and studio – and the client from her workplace in the studio. The entry porch sits neatly between the old and new elements, accessed via timber batten clad gates to both the lane way and the rear garden with a translucent roof above.Save this picture!© Tatjana PlittThe recycled brick wall that encloses the main house and garden runs along one edge of the entry porch separating the public and private areas of the site, the owner from her workplace. The new house is conceived as a simple box with maximum openings to the north, flowing onto bluestone (salvaged from the old garden) paved terraces.All the main spaces are located along the north face of the ground floor, allowing the owner to live entirely on one floor. To protect the north facing glazing from the summer sun, a timber pergola shade structure was designed to sit just beyond the facade line and frame the openings – this extends into the garden on the western side further strengthening the connection with the garden and framing the terrace.Save this picture!First Floor PlanThe simple, efficient and flexible planning of the ground floor allows for a large central kitchen, the heart of the home for the owner who enjoys cooking and entertaining, whilst allowing the main living / dining space to be arranged in a variety of ways. The main bedroom on the ground floor also opens to the north and directly accesses the garden.A large pivot door which separates the main bedroom from the living space can be left opening, effectively joining the spaces and allowing for a full facade of continuous glazing to the north. The maximizing of the passive solar performance of the house along with inclusion of hydronic heating, PV panels, solar hot water system and reuse of materials from the site were crucial considerations.Save this picture!© Tatjana PlittThe upper floor is designed as a distinct, dark timber box sitting atop the ground floor open pavilion element. The form is raked to the south allowing for access to sunlight into the existing studio room over the garage.Timber sun and privacy screening shade the full height north facing windows of the upper bedrooms – framing intimate views of the garden and distant views of the neighbourhood. A roof deck is accessed from the upper floor landing, adding to the outdoor entertaining and potential gardening spaces of the new home, a final square in the ‘  patchwork’  .Project gallerySee allShow lessThe One / gadSelected Projects10 Projects Chosen as Winners of 2017 AIA International Region Design AwardsArchitecture News Share Photographs Projects Garden Wall House / Sarah Kahn ArchitectSave this projectSaveGarden Wall House / Sarah Kahn Architect Photographs:  Tatjana Plitt Manufacturers Brands with products used in this architecture project ShareFacebookTwitterPinterestWhatsappMailOr Clipboard “COPY” 2017center_img Area:  222 m² Year Completion year of this architecture project Save this picture!© Tatjana Plitt Share ArchDaily Year:  ShareFacebookTwitterPinterestWhatsappMailOr Clipboard Australia Houses CopyAbout this officeSarah Kahn ArchitectOfficeFollowProductsWoodSteelBrick#TagsProjectsBuilt ProjectsSelected ProjectsResidential ArchitectureHousesCarlton NorthAustraliaPublished on November 06, 2017Cite: “Garden Wall House / Sarah Kahn Architect” 06 Nov 2017. ArchDaily. Accessed 11 Jun 2021. ISSN 0719-8884Browse the CatalogShowerhansgroheShowers – Croma SelectGlass3MGlass Finish – FASARA™ GradationPartitionsSkyfoldVertically Folding Operable Walls – Zenith® SeriesWall / Ceiling LightsCocowebLighting – Blackspot LED Barn LightUrban ApplicationsIsland Exterior FabricatorsPublic Safety Answering Center II Envelope SystemCeilingsSculptformTimber Batten Ceiling in All Souls ChapelHanging LampsLouis PoulsenLamp – PH 5 + PH 5 MiniGlazedGrespaniaWall Tiles – Porto PetroThermalSchöckInsulation – Isokorb® Concrete to SteelCeramicsTerrealTerracotta Baguettes in Vork CenterCompositesLamitechPlastic facades PanelexCarpetsHalcyon LakeCarpet – Nobsa GreyMore products »Save想阅读文章的中文版本吗?“拼贴布”花园住宅 Garden Wall House / Sarah Kahn Architect是否翻译成中文现有为你所在地区特制的网站?想浏览ArchDaily中国吗?Take me there »✖You’ve started following your first account!Did you know?You’ll now receive updates based on what you follow! Personalize your stream and start following your favorite authors, offices and users.Go to my stream CopyHouses•Carlton North, Australialast_img read more

Young chefs: Culinary Arts students host school, city officials

first_img Remember America’s heroes on Memorial Day “I couldn’t help but think of Jim Smith, a Troy native, the State of Alabama’s Executive Chef, and now a top contender on TV’s ‘Top Chef.’ Jim is a graduate of CHHS and is proof that the culinary arts can lead to large opportunities, even for small town kids.”Wednesday’s luncheon was not the first at the Culinary Arts Academy for City Council Member Wanda Moultry.“The food was delicious and I found the young people inspiring,” Moultry said. “I am always impressed by the skills of these students. I thanked them for inviting me.”Shute said the students who prepared the meal were some of her top students and they did an outstanding job of preparing and serving the meal. Troy falls to No. 13 Clemson Pike County Sheriff’s Office offering community child ID kits Young chefs: Culinary Arts students host school, city officials Published 3:00 am Thursday, January 12, 2017 Latest Stories Email the author Students at the Culinary Arts Academy prepared lunch for members of the Troy City Board of Education and the Troy City Council Wednesday at the Academy building. The students also prepared homemade dressing, rolls and New York-style cheesecake. Inset: Members of the Troy City Council, Wanda Moultry, Stephanie Baker, Greg Meeks and Marcus Paramore enjoyed the lunch in their honor.Students in the Culinary Arts Academy at Charles Henderson High School in Troy put their chef hats on and their culinary arts expertise into play Wednesday as they hosted a luncheon for members of the Troy City Board of Education and the Troy City Council.The month of January is School Board Member Recognition Month and Pam Shute, Culinary Arts Academy instructor, said the students always look forward to preparing meals for guests.“Preparing a meal for guests is a special opportunity for them to put their culinary skills into practice,” Shute said. “For the luncheon honoring the school board members and the members of the city council, the students prepared a meal that began with a chef salad and homemade ranch dressing. The entrée was grilled steak, homemade dressing, twice baked potatoes topped with asparagus, homemade rolls and Southern sweet tea.” Next UpThe meal was topped off with homemade New York-style cheesecake.For Stephanie Baker, a newly elected member of the Troy City Council, the luncheon was a first, a very impressive first.“It was a wonderful dining experience having the students prepare and serve our food,” Baker said. “They were very professional in their service and appearance and the food was delicious. Not only did I enjoy the meal, it was an opportunity to hear the impressive opportunities these culinary students have through this wonderful program at CHHS. Around the WebMd: Do This Immediately if You Have Diabetes (Watch)Blood Sugar BlasterIf You Have Ringing Ears Do This Immediately (Ends Tinnitus)Healthier LivingHave an Enlarged Prostate? Urologist Reveals: Do This Immediately (Watch)Healthier LivingWomen Only: Stretch This Muscle to Stop Bladder Leakage (Watch)Healthier LivingRemoving Moles & Skin Tags Has Never Been This EasyEssential HealthGet Fortnite SkinsTCGThe content you see here is paid for by the advertiser or content provider whose link you click on, and is recommended to you by Revcontent. As the leading platform for native advertising and content recommendation, Revcontent uses interest based targeting to select content that we think will be of particular interest to you. We encourage you to view your opt out options in Revcontent’s Privacy PolicyWant your content to appear on sites like this?Increase Your Engagement Now!Want to report this publisher’s content as misinformation?Submit a ReportGot it, thanks!Remove Content Link?Please choose a reason below:Fake NewsMisleadingNot InterestedOffensiveRepetitiveSubmitCancelcenter_img Book Nook to reopen By Jaine Treadwell Sponsored Content “Presentation is everything and the meal was well presented and the students presented themselves in a very positive manner, she said.Shute said, not only was the meal an expression of appreciation to the school board and city council members for their service to the community, it was also a learning experience for the students and an opportunity for them to showcase their culinary skills.“The preparation of the meal and the opportunity to serve a meal in a rather formal setting was a good learning experience and one that will benefit them in the workplace if they choose to work in the food industry,” Shute said. The students in the Culinary Arts Academy have the opportunity to earn ServSafe Certification.“That certification gives students a better opportunity for employment in the food industry,” Shute said. “An employer doesn’t have to provide them with safety training so, most of the time, a person with a ServSafe certificate will be hired before one without the certification.”Shute is working with Lurleen B. Wallace College in Andalusia and Trenholm State. You Might Like PHOTO OPS: Brundidge native has Christmas encounter with president Brundidge native Lieutenant Colonel Letitia Bryant and her children were among 300 service members and their families who were selected… read more By The Penny Hoarder The Penny Hoarder Issues “Urgent” Alert: 6 Companies… Print Article Plans underway for historic Pike County celebrationlast_img read more

Flowers spark memory

first_imgLatest Stories Remember America’s heroes on Memorial Day Penny Hoarder Issues “Urgent” Alert: 6 Companies Are… Skip Print Article Sponsored Content Charlotte and I were Den Mothers for Cub Scout Den 34.  When we retired, we each got a sliver tray.  We deserved Purple Hearts.These little boys were only Cub Scouts. They knew nothing of the Scout Law, therefore, nothing about being helpful, friendly, courteous, kind, obedient and cheerful.So, daffodils remind me of the fisticuffs that broke out in the back of a pickup truck on the way back from Camp Alaflo. One of the Cubs bolted from the truck and took off running across the pasture, sending cows running helter-skelter. Had the fleet little feller not come face-to-face with a snorting bull, he would have been in Mississippi by nightfall. Flowers spark memory Published 3:00 am Saturday, February 25, 2017 By The Penny Hoarder Driving through the Pike County countryside, the sight of daffodils nodding greetings makes my heart both sing and shudder. Sing, from the memory of the small bouquet of the yellow flowers loosely arranged in a Mason jar on my grandmother’s kitchen table.Shudder, from the memory of daffodils arranged in Mason jars on the table at the Cub Scouts’ February Blue and Gold Banquet.    Book Nook to reopen Email the author Daffodils bring memories of the yellow jackets that swarmed up my culottes while attempting to rescue a Cub from their nest. Daffodils remind me that Cubs dressed-up in girls’ clothing and wearing earbobs, lipstick and red, red rouge can still ball up their fists and fight.I shudder when I think of trying to rescue a Cub who couldn’t swim while trying to stay afloat in black water wearing a panty girdle. I shudder at the memory of the Pack’s formal social and dance.    By Jaine Treadwell You Might Like Troy right to deny solar project Last week, the Troy City Council and Troy Board of Adjustments effectively squashed Eagle Solar Group’s plans to build a… read more Plans underway for historic Pike County celebration The idea of dates was not met with any measure of enthusiasm by the parents. The Cubs were too young  for such a thing.  And, the little girls’ parents were adamant. Ridiculous! Scandalous! We, the fearless leaders, prayed in earnest that none of the girls would get in the family way before they were forty or we would be to blame.Shudder at the memory of Cub Scout Sports Day. We loaded the Cubs in my Ford Station Wagon that was a long as a Greyhound bus and took off for Dothan. First we played putt-putt golf, then we went roller skating. For the closing activity we went to the bowling alley. It has been a long sporting day but the Cubs were not ready to hibernate. In fact, they were rowdy. Before we could ever get out of the parking lot at the bowling alley, fights broke out in the back of the station wagon. Charlotte screamed like a banshee, got on her knees in the front seat and with her glasses on the end of her nose and her eyes glaring, she gave the Cubs a verbal lashing. Why, she used words I had never heard. When she finished, you could have heard a pin drop in that old Ford station wagon. Not a muscle moved.When she was done, she pushed her glasses back up on her nose and quietly said, “Now, I don’t think the Boy Scouts of America would approve of the language I’ve used tonight ….” Daffodils. They make my heart shudder – and sing.Jaine Treadwell is features editor of The Messenger. Contact her at [email protected] Troy falls to No. 13 Clemson Pike County Sheriff’s Office offering community child ID kits Around the WebIf You Have Ringing Ears Do This Immediately (Ends Tinnitus)Healthier LivingMd: Do This Immediately if You Have Diabetes (Watch)Blood Sugar BlasterHave an Enlarged Prostate? Urologist Reveals: Do This Immediately (Watch)Healthier LivingWomen Only: Stretch This Muscle to Stop Bladder Leakage (Watch)Healthier LivingRemoving Moles & Skin Tags Has Never Been This EasyEssential HealthRemember Them? I’m Sure Their New Net Worth Will Leave You SpeechlessbradofoThe content you see here is paid for by the advertiser or content provider whose link you click on, and is recommended to you by Revcontent. As the leading platform for native advertising and content recommendation, Revcontent uses interest based targeting to select content that we think will be of particular interest to you. We encourage you to view your opt out options in Revcontent’s Privacy PolicyWant your content to appear on sites like this?Increase Your Engagement Now!Want to report this publisher’s content as misinformation?Submit a ReportGot it, thanks!Remove Content Link?Please choose a reason below:Fake NewsMisleadingNot InterestedOffensiveRepetitiveSubmitCancellast_img read more

USU Men’s Basketball Faces Montana State Tuesday Evening

first_img Written by Brad James FacebookTwitterLinkedInEmailLOGAN, Utah-Tuesday evening, No. 17 Utah State men’s basketball commences its 115th season by hosting Big Sky Conference foe Montana State at the Spectrum.The Aggies are 84-30 (.737) all-time in season openers and have won 23 of their last 25 such games.Dating back to the 1957-58 season, Utah State is 53-9 (.852) all-time in home openers.In the last 10 meetings between the Aggies and the Bobcats, the team that has had a better shooting percentage has won the game.Utah State will be led by reigning Mountain West Conference player of the year and Mountain West Conference MVP, senior guard Sam Merrill.The former Bountiful High School star posted 20.9 points and 4.2 assists last season to lead the squad.Portuguese national, sophomore center Neemias Queta, averaged 11.8 points and 8.9 rebounds last season for the Aggies.The Bobcats come into Tuesday’s game, having routed Yellowstone Christian 94-43 at Worthington Arena of Bozeman, Mont.Montana State’s leading returning scorer is senior guard Harald Fry. The Norwegian national averaged 17.2 points and 4.5 rebounds per game last season for the Bobcats.The Aggies lead the Bobcats 78-40 all-time in a series dating back to the 1913-14 season. Tags: Montana State Men’s Basketball/USU Men’s Basketball November 5, 2019 /Sports News – Local USU Men’s Basketball Faces Montana State Tuesday Eveninglast_img read more


first_imgDownload arrest warrantsFacebookTwitterCopy LinkEmailShare LINK TO VANDERBURGH COUNTY ARREST WARRANTS FOR JUNE 17, 2016last_img

New firm offers European craft

first_imgA start-up company, The Cotswold Food Partnership, is targeting supermarkets and craft bakers with new ranges of bake-off artisan and functional breads.The company has sourced its range from European craft bakeries in countries including Holland and Germany and it also has a manufacturing partner, the BRC-accredited Scottish craft baker Fords the Bakers, Lothian.Carl Le Neveu, founder of the company, told British Baker: “There’s a gap in the market – the consumer demand is there, but the products aren’t readily available.”The functional breads on offer will include a white loaf with all the nutrition and fibre of a wholewheat, and a ’hearty’ loaf, developed with the Dutch Heart Foundation. And on the Continental breads side, Le Neveu said high-quality artisan breads were on offer.He added: “The UK’s demographic has changed dramatically over the past decade. For example, there are three quarters of a million Polish workers in the UK. We’re talking to major retailers about offering breads such as Polish Rye on a regional basis.”The primary target for the part-baked frozen breads is in-store bakeries said Le Neveu, but the company is also developing a range of mixes for the craft bakery sector.last_img read more

Possible early Costa demerger, says analyst

first_imgSpeculation surrounding the possibility of Costa Coffee demerging from the Whitbread Group is mounting, according to a City analyst.It comes as the coffee chain announced the appointment of Chris Rogers, the group’s current financial director, as the brand’s new managing director on Tuesday (3 April). He will replace John Derkach, who is due to step down from his position on 1 August to join UK restaurant business Tragus Group as chief executive.A spokesperson from Deutsche Bank AG, Filiale London, said: “This move is likely to prompt two responses: relief that he is not leaving Whitbread and speculation that Costa is being lined up for demerger. We have written extensively that we see Costa remaining with the group for some time, so shareholders can benefit from the significant growth expected from the plan to double the estate size by 2016E.“We have an EBITDA forecast of £175m for that year, and if demerger was a near-term option, why is the managing director leaving? We see an early demerger of Costa as potentially limiting the realisable value from the Costa growth plans for investors.”Speaking of the current MD’s work at Costa, the representative from Deutsche Bank added that Derkach has managed to more than triple sales and quadruple profits.Ahead of the company’s 2011/12 financial results, to be announced on 26 April, the City analyst said: “At Costa, we are looking for 26% top line growth, and with margins forecast to rise +75 bps, we are focusing on EBITDA of +35%. We estimate EBITDA should reach £97m in the year just ended.”last_img read more

Blog: Every little helps, or not?

first_imgIs having the cash from a national name to back your brand the key to grow, or are bakery retailers best off funding their own businesses?As many have pondered in recent months, Tesco’s 49% investment in artisanal coffee shop business Harris + Hoole has resulted in an amalgamation of “Beware” signs posted online and in the national press.“The trendy chain of posh coffee shops that’s Tesco in disguise: Pop in for a latte and it looks like a quirky family business,” reads a recent article on the Daily Mail website.While the words ‘Tesco’s 49% investment’ have been bolded and underlined again and again in news stories, I wonder whether this taints the general public’s perception of an independent company, particularly this one set up by the Aussie siblings behind Taylor Street Baristas.I speak to two ladies, probably in their sixties, passing me on the local high street in Pinner, north-west London. They are stood with Wenzel’s the Bakery on one side of them – a local, independent and family-run business – and the latest Harris + Hoole outlet on the other.“Which out of the two do you prefer?” I ask the women. One responds with: “[Harris + Hoole] seems a bit too dark inside, and have you seen the price of their tea?”Brushing off the aesthetic-themed answers, I ask a second question: “Do you know Tesco have plumped a large investment in this business? In fact, they have a 49% stake in it.” I’m greeted with blank looks and a chorus of female laughter. “No dear, that’s not the problem,” is the final answer I receive as they shuffle off together.Upon stepping inside the latest branch of the now 10-store-strong estate, I get the impression that this does not bother the hoards of customers seated inside and queuing up at the till. Just after the lunchtime rush, around 2pm, a mixture of yummy mummys, college students, couples, OAPs and local folk have filled the last of the empty chairs and tables. Whether people know the Tesco tag is attached to the front door, or if people are blissfully unaware of the supermarket’s involvement. It does not seem to be putting off customers from pursuing their everyday coffee and cake rituals at Harris + Hoole.While the media hype behind this investment has evidently not put off coffee drinkers from visiting the establishment, I wonder whether the idea still deters many of our UK high street craft bakeries from leaping at an opportunity to partner up with a supermarket giant, or any other nationally-known financial investor, should the opportunity arise.Is there a stigma attached to such a deal that you and your business would be classed as a sell-out? Or it does it mean that your business could flourish and take the hassle out of the cash behind success?To balance the negative media hype played out in such a dramatic fashion, the Aussie coffee connoisseurs took a risk with Tesco partnership and the steady stream of customers should now be that peace of mind that their gamble has paid off. Ultimately what all businesses are looking for in the current rocky economic climate is getting people through the door. If the public was so put off by the concept then, frankly, Harris + Hoole would have been the next high street casualty of many currently marring the bleak retail landscape.last_img read more

Listen To Greensky Bluegrass’s Incredible Telluride Show With Jerry Douglas & Sam Bush

first_imgBeloved group Greensky Bluegrass continues to turn heads with their infectiously soulful brand of bluegrass. The five-piece from Michigan made their way West not too long ago, finding themselves at the esteemed Telluride Bluegrass Festival on June 17th. There, the group played their hearts out, jamming with bluegrass legends like Jerry Douglas and Sam Bush during their set.The set also featured a unique tribute to both Prince and David Bowie, as Greensky played Bowie’s “Fame” in the middle of their long-played cover of Prince’s “When Doves Cry.” It was a truly great celebration of music all around.Thanks to a taper named david, we have full audio from this beautiful set. Tune in below and enjoy!Setlist: Greensky Bluegrass at Telluride Bluegrass Festival, Telluride, CO – 6/17/16Set: Leap Year, Wings for Wheels, Demons, Instrumental* > The Ballad of Curtis Loew* > Don’t Lie*, Lose My Way*%, Frederico*%, Windshield > Take Cover, When Doves Cry > Fame > When Doves Cry, Burn Them* = w/ Jerry Douglas% = w/ Sam Bush[Photo by Dylan Langille]last_img read more

Promising projects

first_img NIH makes $8.5M investment in promising projects Eight Harvard scientists receive funding through High Risk, High Reward program Seven recognized for high-risk, high-reward research Sixteen Harvard scientists are among the 93 researchers who have been selected to receive grants through the National Institutes of Health’s High-Risk, High-Reward Research Program, which funds innovative study addressing major challenges in biomedical science.Jason Buenrostro, assistant professor of stem cell and regenerative biology; Brian Liau, assistant professor of chemistry and chemical biology; Sichen Shao, assistant professor of cell biology; Brian Edlow, assistant professor of neurology, Massachusetts General Hospital (MGH); Rajat Gupta, instructor of medicine at Harvard Medical School and a cardiologist at Brigham and Women’s Hospital; Ryuji Morizane, assistant professor of medicine, MGH; Seth Rakoff-Nahoum, assistant professor of pediatrics, Boston Children’s Hospital; Alexandra-Chloé Villani; assistant professor of medicine, MGH; and Courtney Yuen, instructor in medicine, Brigham and Women’s, will receive New Innovator Awards.Vadim Gladyshev, professor of medicine, Brigham and Women’s, will receive a Transformative Research Award.Michael Mina, assistant professor of epidemiology; Kapil Ramachandran, a junior fellow of the Harvard Society of Fellows and a research associate in molecular and cellular biology; and Sol Schulman, instructor in medicine, Beth Israel Deaconess Medical Center (BIDMC), will receive Early Independence Awards.Mark Andermann, associate professor of medicine, BIDMC; Sun Hur, associate professor of biological chemistry and molecular pharmacology and associate professor of pediatrics at Boston Children’s; and Hidde Ploegh, a member of the faculty of pediatrics at Boston Children’s, will receive NIH Director’s Pioneer Awards.“Each year, I look forward to seeing the creative approaches these researchers take to solve tough problems in biomedical and behavioral research,” said NIH Director Francis S. Collins. “I am confident the 2019 cohort of awardees has the potential to advance our mission of enhancing health through their groundbreaking studies.”Jason Buenrostro is interested in the ways that adult stem cells can harbor epigenetic errors, and how these relatively minute changes can lead to large changes in the cell’s normal capacity to self-renew and differentiate.Sometimes called epi-mutations, those errors don’t result in changes to a cell’s DNA sequence, but rather produce changes in the expression of specific genes.Buenrostro plans to use the NIH funding to develop a model for understanding how errors associated with maintaining an epigenetic state, and the propagation of these errors to daughter cells, could be a key missing piece in our understanding of how and why cells make certain fate decisions.“Traditional methods used to study the epigenome … lack the resolution to measure epigenomic dynamics in human tissues,” Buenrostro explained. “We want to develop new methods to measure the epigenome at single-cell resolution and apply these methods to primary hematopoietic stem cells.”That understanding, he said, won’t just shed new light on how epigenomic alterations may alter lineage potential of hematopoietic stem cells in normal and diseased states, but could also provide powerful insights into therapeutic targets.Buenrostro’s lab also plans to expand their experimental tool kit, which will empower research efforts that seek to understand regulatory changes across diverse cell types, which may ultimately help guide the direction of therapeutics that seek to intervene in disease progression.Brian Liau’s lab is devoted to deciphering the structure-function relationships between chemical inhibitors and their biological targets. That understanding, he said, is essential not only for target validation and lead compound optimization, but also for understanding drug mechanism of action as well as target biology.“I am thrilled to receive this grant, which was a pleasant surprise,” Liau said of the award. “Receiving this grant has been a tremendous boost to my lab, providing invaluable resources that allow us to more freely pursue our most ambitious ideas.”One of those ideas, Liau said, is the development of new approaches that would combine chemical inhibitor profiling with genome editing to systematically alter proteins to probe those relationships directly in cells in a high-throughput manner. They are currently applying these strategies to study the functions of key proteins that regulate gene expression.Developing high-throughput biochemistry tools could provide novel perspectives and insight into protein structure and function as they relate to the small-molecule mechanism of action, and could prove critical for elucidating disease mechanisms and developing novel small-molecule therapies.Sichen Shao’s lab is focused on understanding how cells monitor different steps of protein biosynthesis to maintain a high-quality proteome. That process is critical, she said, because failing to correctly make proteins or degrade defective proteins is the root of many genetic, neurodegenerative, and aging-related diseases.“Understanding how cells distinguish rare aberrant products from similar biosynthetic intermediates can reveal how mistakes at the molecular level lead to disease and identify new therapeutic targets,” Shao said.To understand how cells troubleshoot the process of protein synthesis, Shao’s work has turned to a host of tools, including biochemical reconstitutions, cell biological assays, and structural studies, to decipher the mechanisms that detect and handle problems encountered by ribosomes, the cellular machines that synthesize proteins.The New Innovator Award, she said, will support efforts to generate high-throughput experimental tools and establish a “ribosome recognition” code defining the factors that recognize different types of aberrant ribosomes and propagating distinct cellular responses to regulate gene expression and protein homeostasis.Brian Edlow’s lab at the MGH Center for Neurotechnology and Neurorecovery is developing tools to detect, predict, and promote recovery of consciousness in patients with severe traumatic brain injury.Rajat Gupta’s research focuses on identifying new treatments for vascular disease using human genetics to discover the causal biologic pathways.Ryuji Morizane has pioneered research in stem cell differentiation and kidney organoids. He directs research groups focused on kidney regenerative medicine, genome editing in stem cells, and kidney disease modeling with kidney organoids.Seth Rakoff-Nahoum’s lab studies how each member of a microbiome interacts with the environment, each other, and the host, and uses genetic, molecular, cellular, and computational approaches combined with ecological and evolutionary theory to address these questions.Alexandra-Chloé Villani’s lab aims to achieve higher-resolution definition and functional characterization of cell subsets and rules governing human immune-response regulation as a foundation for deciphering how immunity is dysregulated in diseases and for developing a comprehensive human immune lexicon that is key effectively translating findings from bench to bedside.Courtney Yuen’s work focuses on improving the detection and prevention of tuberculosis and evaluating the impact of interventions in this area. She has collaborated with government and nongovernment programs in Kenya, Peru, Pakistan, and the U.S.Vadim Gladyshev seeks to define principles of lifespan control and use this information to develop interventions that extend a person’s life.Over the past two years, Michael Mina has worked with Stephen Elledge, Gregor Mendel Professor of Genetics and HHMI Professor at Harvard Medical School and Brigham and Women’s, to further refine a technology that can simultaneously measure and identify all of the antibodies that a person has against nearly any infectious pathogen using less than a single drop of blood.Although the technology can provide new insights into an individual’s immunological history, Mina said a larger goal is to use the system to learn how infectious diseases spread and use the data, for example, for early epidemic or outbreak detection.“You can think of the immune system, and in particular here the antibody repertoire, as an encrypted hard drive,” he said. “Once we decrypt it, it provides a tremendous level of potentially time-stamped details regarding infections, vaccines, and much more.”The NIH funding, Mina said, will allow him to build a lab that is focused on developing and applying new tools to read and interpret immunological repertoires and couple them with mathematical models to improve global public health efforts surrounding infectious disease surveillance, novel pathogen detection, and vaccine monitoring.“My overarching goal is to develop ‘bio-computational’ tools centered on unlocking the troves of information contained within the antibody repertoire to be applied to infectious disease epidemic and emerging pathogen outbreak detection, to improving global public health surveillance, and to helping to improve how and when vaccines are given over a lifetime,” he said.Kapil Ramachandran plans to use the NIH grant to further investigate his discovery of a new pathway of neuronal communication that also keeps neurons healthy.For years, he said, it has been understood that protein complexes called proteasomes act as a form of intracellular “trash removal,” breaking down unneeded proteins into smaller fragments called peptides. Ramachandran, however, discovered a variant of those proteasomes that sits at the interface between the inside and outside of neurons.“In this way, when this neuro-proteasome degrades a protein, it actually immediately releases the degradation fragments which go on to signal to other cells in the brain,” he said. “This is profoundly unusual, because it suggests that there is an entire system of communication that we have been missing for a long time.”Ramachandran’s plan is to use genetic and molecular tools to dissect that pathway to understand how that system of degradation and signaling is coded and decoded in the brain, and to understand whether and how the pathway may be related to a number of neurodegenerative and neurodevelopmental disorders.“I’m absolutely thrilled to have received this award,” he said. “This allows us to accelerate our work in ways that I have dreamed about for three years, and it puts into motion a tremendous amount of built-up potential energy on my work.”Sol Schulman’s lab integrates functional genetics, genomics, protein biochemistry, and cell biology to identify new mechanisms regulating the initiation of blood coagulation relevant to the pathophysiology and treatment of human bleeding and thrombotic disease.Mark Andermann’s lab seeks to understand how the needs of the body bias learning, attention, and imagery toward need-relevant objects, and how our attention shifts from these external stimuli toward internal body signals. To achieve these goals, the lab employs cellular and subcellular imaging methods to track the activity of specific brain cells in the retina, thalamus, cortex, amygdala, hypothalamus, and brainstem across weeks as mice seek food, water, mates, or safety. A better understanding of the fundamental mechanisms by which the brain and body communicate is of broad relevance to psychiatry, neurology, and medicine.Sun Hur is interested in biochemical and structural mechanisms of protein-nucleic acid interactions in the immune system. These include innate immune receptors that recognize foreign nucleic acids, and transcription factors that play important roles in T cell development.Hidde Ploegh’s focus is the biochemistry of immune recognition, in particular mechanisms by which pathogens and tumors avoid detection by the immune system. He is known for his analysis of the pathways involved in antigen processing and presentation by products of the major histocompatibility complex (MHC); for studies of glycoprotein synthesis, turnover, trafficking, and quality-control mechanisms; and for pioneering the use of human leukocyte antigen (HLA) transgenic mice to examine the properties of human MHC products as restriction elements. He has applied peptide chemistry to develop probes to study the activity of the proteasome and ubiquitin-specific proteases, and has utilized bacterial sortases for novel protein engineering applications. He has employed these technologies in the generation of improved cytokines, and most recently in conjunction with the isolation of camelid-derived single-domain antibodies, in the creation of improved tools for cytofluorimetry and noninvasive visualization of antitumor and antivirus immune responses using positron emission tomography.The NIH issued 11 Pioneer awards, 60 New Innovator awards, nine Transformative Research awards, and 13 Early Independence awards for 2019. The 93 awards total approximately $267 million over five years, pending available funds. Funding for the awards comes from the NIH Common Fund; National Center for Complementary and Integrative Health; National Institute of Biomedical Imaging and Bioengineering; National Institute of General Medical Sciences; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; National Institute on Aging; National Institute on Alcohol Abuse and Alcoholism; and National Institute on Drug Abuse. Unraveling the brain’s secretscenter_img Harvard scientists receive grant funding through NIH program Related Harvard researchers among those receiving more than $150 million in funding from the NIH BRAIN Initiative last_img read more